Figure 2
Optimization of Cell Dose and Incubation Time to Determine the Cord Blood Stem Cell Response for TNC and MNC Fractions. A. Proliferation Potential of CFC-GEMM and HPP-SP Present in Either TNC or MNC Cultured at High Cell Concentrations (2,500, 5,000, 7,500 cells/well) for 5 Days. Dotted lines represent the TNC fraction, while solid lines represent the MNC fraction. Cell dose response linear regression parameters for N = 22. TNC, CFC-GEMM: Slope = 1.18 × 10-5, r = 0.99. TNC, HPP-SP: Slope = 1.84 × 10-5, r = 0.99. MNC, CFC-GEMM: Slope = 3.69 × 10-5, r = 0.99. MNC, HPP-SP: Slope = 4.92 × 10-5, r = 1.0. Probability that the slopes of the linear regression curves are different. TNC CFC-GEMM vs TNC HPP-SP, P = 0.1. MNC CFC-GEMM vs MNC HPP-SP, P = 0.007. TNC CFC-GEMM vs MNC CFC-GEMM, P = 0.02. TNC HPP-SP vs MNC HPP-SP, P = 0.05. Two-way ANOVA between the different response curves shown on the graph as P values. B. Proliferation Potential of CFC-GEMM and HPP-SP Present in Either TNC or MNC Cultured at Low Cell Concentrations (1,000, 2,000 and 4,000 cells/well) for 7 Days. Dotted lines represent the TNC fraction, while solid lines represent the MNC fraction. Cell dose response linear regression parameters for N = 41. TNC, CFC-GEMM: Slope = 4.38 × 10-5, r = 1.0. TNC, HPP-SP: Slope = 8.04 × 10-5, r = 0.99. MNC, CFC-GEMM: Slope = 0.00016, r = 1.0. TNC, HPP-SP: Slope = 0.00023, r = 1.0. Probability that the slopes of the linear regression curves are different. TNC CFC-GEMM vs TNC HPP-SP, P = 0.34. MNC CFC-GEMM vs MNC HPP-SP, P = 0.0008. TNC CFC-GEMM vs MNC CFC-GEMM, P = 0.03. TNC HPP-SP vs MNC HPP-SP, P = 0.02. Two-way ANOVA between the different response curves shown on the graph as P values.