Figure 1From: Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units Correlation between the CFU Differentiation and Proliferation Assays for TNC and MNC Cord Blood Fraction. A. The Difference in Total CFU Colony Counts for CFC-GEMM Stem Cell Population Enumerated for TNC and MNC Fractions by Two Investigators (P1 and P2). Cell dose response linear regressions. TNC, P1: Slope = 0.0095, r = 0.895. MNC, P1: Slope = 0.0230, r = 0.922. TNC, P2: Slope = 0.0062, r = 0.89. MNC, P2: 0.016, r = 0.91. Probability of the linear regression slopes being equal?. TNC P1 vs MNC P1, P = 0.0009. TNC P2 vs MNC P2, P = 0.0008. TNC P1 vs TNC P2, P = 1.0. MNC P1 vs MNC P2, P = 0.096. TNC P1 vs MNC P2, P = 0.03. TNC P2 vs MNC P1, P = 0.0001. Two-way ANOVA to determine differences between TNC vs MNC and P1 vs P2. TNC P1 vs MNC P1, P = 0.0002. TNC P2 vs MNC P2, P = 0.02. TNC P1 vs TNC P2, P = 0.19. MNC P1 vs MNC P2, P = 0.0001. TNC P1 vs MNC P2, P = 0.0001. TNC P2 vs MNC P1, P = 0.33. B. Correlation Between Total CFU Colony Counts for CFC-GEMM Enumerated on Day 10 and CFC-GEMM Proliferation Detected by ATP Bioluminescence on Day 7. Cell dose response linear regressions for 1,250, 2,500 and 5,000 cells/well. TNC, P1: Slope = 0.0137, r = 0.99. MNC, P1: Slope = 0.017, r = 0.99. TNC, P2: Slope = 0.0086, r = 0.99. MNC, P2: Slope = 0.015, r = 0.99.Back to article page