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Figure 1 | Journal of Translational Medicine

Figure 1

From: Detecting primitive hematopoietic stem cells in total nucleated and mononuclear cell fractions from umbilical cord blood segments and units

Figure 1

Correlation between the CFU Differentiation and Proliferation Assays for TNC and MNC Cord Blood Fraction. A. The Difference in Total CFU Colony Counts for CFC-GEMM Stem Cell Population Enumerated for TNC and MNC Fractions by Two Investigators (P1 and P2). Cell dose response linear regressions. TNC, P1: Slope = 0.0095, r = 0.895. MNC, P1: Slope = 0.0230, r = 0.922. TNC, P2: Slope = 0.0062, r = 0.89. MNC, P2: 0.016, r = 0.91. Probability of the linear regression slopes being equal?. TNC P1 vs MNC P1, P = 0.0009. TNC P2 vs MNC P2, P = 0.0008. TNC P1 vs TNC P2, P = 1.0. MNC P1 vs MNC P2, P = 0.096. TNC P1 vs MNC P2, P = 0.03. TNC P2 vs MNC P1, P = 0.0001. Two-way ANOVA to determine differences between TNC vs MNC and P1 vs P2. TNC P1 vs MNC P1, P = 0.0002. TNC P2 vs MNC P2, P = 0.02. TNC P1 vs TNC P2, P = 0.19. MNC P1 vs MNC P2, P = 0.0001. TNC P1 vs MNC P2, P = 0.0001. TNC P2 vs MNC P1, P = 0.33. B. Correlation Between Total CFU Colony Counts for CFC-GEMM Enumerated on Day 10 and CFC-GEMM Proliferation Detected by ATP Bioluminescence on Day 7. Cell dose response linear regressions for 1,250, 2,500 and 5,000 cells/well. TNC, P1: Slope = 0.0137, r = 0.99. MNC, P1: Slope = 0.017, r = 0.99. TNC, P2: Slope = 0.0086, r = 0.99. MNC, P2: Slope = 0.015, r = 0.99.

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