ATGL and PEDF co-localize with lipid droplets in cardiomyocytes. A: cardiomyocyte lysates were immunoprecipitated with ATGL or PEDF antibody, and the lysates were then detected with PEDF or ATGL antibodies. B: co-localization of PEDF (green) and ATGL (red) assessed via the double immunofluorescent labeling of cardiomyocytes. Yellow color indicates the merging of green and red. Scale bar, 2 μm. C: immunoelectron microscopy images showed that PEDF (labeled with gold particles of 5 nm in diameter) and ATGL (labeled with gold particles of 10 nm in diameter) co-localized around the lipid droplet and that the spatial distance between PEDF and ATGL was less than 20 nm. Scale bar, 100 nm.