Figure 5

Quantitative-stereological analyses of pancreata from liraglutide- and placebo-treated GIPR ^dn transgenic pigs. Representative histological pancreas sections of (A) immunohistochemical staining for insulin and (D) glucagon as well as (G) double immunohistochemical staining for Ki67 (blue) and insulin (brown) of liraglutide- (L) and placebo-treated (P) GIPR^dn transgenic pigs, red arrow: proliferating beta-cell, white arrow: proliferating acinus-cell, scale bar = 50 μm. (B) Total beta-cell volume [V_{(beta-cell, Pan)}] and (C) total beta-cell volume related to body weight [V_{(beta-cell, Pan)}/BW] as well as (E) total alpha-cell volume [V_{(alpha-cell, Pan)}] and (F) total alpha-cell volume related to body weight [V_{(alpha-cell, Pan)}/BW]. (H) Beta-cell proliferation rate and (I) acinus-cell proliferation rate. Data are means ± SEM, *: p < 0.05, **: p < 0.01.