Figure 2

Printing of RPMA. A, Scheme of RPMA printing processed for anti-LDH-A is shown magnified. One nl samples were spotted in quadruplicate. Black boxed: negative controls of BSA; Blue boxed: standard curves of HCT116 cells; Green boxed: tissue samples from control donors; Red boxed: tissue samples from patients. Below are shown representative RPMAs processed with other antibodies. B, The plot illustrates the linear correlation that exists between the fluorescence intensity (arbitrary units, a.u.) and the amount of LDH-A protein in HCT116 cell lysates. Protein concentrations in the biopsies were calculated according to the fluorescence intensity obtained in the linear plot of HCT116 cells. For other details see Additional file 2: Figure S2.