mASC and mASC-CM effect on neutrophil numbers and activity. Total WBC (T, grey) and PMN (N, white) counts in BALF were significantly reduced by mASC (A), but not mASC-CM (B). The ratio between macrophages (M), lymphocytes (L) and neutrophils (N) in BALF of LPS-treated mice was not significantly affected by either experimental treatment. N = 5-6 animals per group. (C) WBC from BALF of LPS-challenged mice (N = 6) were subjected to vehicle (LPC cn) or 1 μg/ml LPS stimulation (LPS st). In parallel, WBC from BALF of LPS/ASC-CM-treated mice (N = 6) were subjected to vehicle control (LPS/CM cn) or LPS stimulation (LPS/CM st). LPS-induced ROS generation by WBC from BALF of LPS/ASC-CM mice was significantly lower comparing to WBC from BALF of LPS/media mice. T-test was used to analyze the significance of differences between groups.