Elevated expression of STC1 is associated with metastasis of T1 stage ccRCC. Effects of STC1 interferences on cell proliferation. (A) Analysis showing higher STC1 expression in T1 stage metastatic tumors when compared with localized tumors. (B) Analysis showing significantly larger average diameter in metastatic tumors (5.433 ± 0.644 cm, n = 10) when compared with localized tumors (3.833 ± 1.187 cm, n = 96). (C) Positive correlation of STC1 mRNA levels and average tumor diameter in T1 stage (n = 106, r = 0.215, p = 0.027). (D) (E) Alteration for STC1 mRNA and protein levels in Caki-1-Metastatic cell line, A498-Non-metastatic cell line and Caki-2-Non-metastatic cell line cells after knockdown or overexpression. (F) MTS assay showed STC1 knockdown reduced the proliferation of Caki-1 and A498 cells, while STC1 overexpression promoted the proliferation of Caki-2 cells. (G) (H) Effects of STC1 interferences on anchorage-dependent and independent growth of RCC cells. *,P < 0.05; **,P < 0.01; ***,P < 0.001.