Figure 4From: Analytical platform evaluation for quantification of ERG in prostate cancer using protein and mRNA detection methods Detection of ERG protein using ELISA. A. Evaluation of ERG antibody reactivity in the ELISA format. B. Capture and detection antibody combinations for development of antigen capture assay (sandwich ELISA). C. The limit of detection of purified recombinant ERG protein was 30Â pg. D. The limit of detection of ERG protein from VCaP cell lysates (without mixing with LNCaP cells) was 10,000 cells.Back to article page