CLE-induced glucose uptake in fully differentiated L6 myocytes is dependent on the PI3K pathway, but not the AMPK pathway. L6 cells were treated with CLE with or without compound C (20 μM) or LY294002 (20 μM) for 30 min, and then assayed for glucose uptake using 2-NBDG, a fluorescent derivative of glucose as a glucose analog. Glucose uptake was measured using a fluorescence reader. Data are presented as means ± SE (n = 3). *
p < 0.05 and **
p < 0.01 versus control.