Figure 4

Biologic functions of Alanine on gastric and breast cancer cell lines in vitro . (A-C). Cell viability was assessed by CCK-8 proliferation assay for indicated gastric cancer and breast cancer cells at 24 h (A), 48 h (B) and 72 h (C) after treatment with 10 mM Ala or negative control medium (NC). (D-I). Dynamic changes of cell numbers were measured by Cell-IQ system. The numbers of total cells (D), stable cells (E) and differentiated cells (F) from SGC-7901 cell line treated with 10 mM Ala or not. The numbers of total cells (G), stable cells (H) and differentiated cells (I) from MDA-MB-231 cell line treated with 10 mM Ala or not. Ratios of cell numbers were calculated by formula: [values at indicated time point – values at initial time point]/values at initial time point. Data were presented as means ± SD of three independent experiments. Every CCK-8 assay was performed in quintuplicate and 24 measurements were evaluated in each group for Cell-IQ assay. *P < 0.05 versus NC.