Figure 3

Effects of AD-MSCs on functional recovery, lesion size and cell death after pMCAO. Functional outcome was measured by Roger’s test after pMCAO (A). Lesion size was measured using in vivo MRI at 24 h and 14 d (B) and H&E at 14 d (C). MAP-2 (4×) and GFAP (20×) staining was used to confirm the lesion zone (D). Cell death was evaluated by TUNEL staining in the peri-infarct zone at 14 d (40×) (E) (scale bars = 20 μm). Results are presented as mean ± SD (n = 10 per group) (p < 0.05).