Figure 1From: Identification of stroke-associated-antigens via screening of recombinant proteins from the human expression cDNA library (SEREX) 1st screening by SEREX and Western blot analysis. Recombinant proteins were blotted onto NitroBind nitrocellulose membranes and reacted with patient plasma (arrow head). (A) Affinity-purified GST-tagged antigens were separated on 12% SDS-polyacrylamide gels stained with Coomassie staining (lane 1), or Western blotted using anti-GST antibody (lane 2) or autologous sera (lane 3) (B).Back to article page