Figure 2

Morphological changes and cell cycle perturbation by barasertib-HQPA exposure. A. Increase in HBL cell size induced by barasertib-HQPA exposure at 300 nM by light inverted microscopy. B. Cells were incubated, for one and three days, with barasertib-HQPA (30 and 300 nM) and the cell cycle was analysed by flow cytometry analysis as described in M & M section. Experiments were performed in triplicate obtaining similar results and histograms are representative for all of them. Results of 3 days-exposure are reported in the inserts.