Figure 1

Determinants of resistance to vemurafenib. A. Cell cycle progression of the two pair of cells, MBA72/ MBA72R and Hmel-1/Hmel-1R, were determined as described in M&M section. B. p-Erk1/2, Erk1/2, p-Akt, Akt, p-p38 MAPK and p38 MAPK were analysed in cells by western blotting and protein amount determined by densitometry analysis. Histograms are means of at least three different experiments. *p < 0.05 vs untreated cells. C. The expression level of MITF was determined by FCM in MBA72, MBA72R and HBL as respect to the isotype.