BBR ameliorates hepatic steatosis in HFD-fed rats. (A)The livers isolated from rats fed with a normal diet (ND), high-fat diet (HFD) or HFD plus Berberine (HFD + BBR)were stained with hematoxylin and eosin. Photographs are at 200 × magnification. A large magnification is shown in the white box. Ballooning cells and Mallory bodiesare shown in black and red arrows, respectively. (B) Hepatic TG contents, and (C) serum TC and LDL-c levels were measured in the experimental animals as described in “Methods”. Data are mean ± SEM. * p < 0.05, ** p < 0.01 versus ND; # p < 0.05 versus HFD (assessed by one-way ANOVA followed by Tukey’s multiple comparison tests). BBR, berberine; HFD, high-fat diet; ND, normal diet; TG, triglyceride; TC, cholesterol.