Figure 5

ZBP-89 decreased HDAC3 but increased Bak in xenograft tumor tissue. Ad-ZBP-89 infection effectively enhanced ZBP-89 levels in 10 tumor-bearing mice. The expression of HDAC3 in xenograft tumor tissues was examined by both immunohistochemical staining and Western blotting analysis (a and c). The positive-stained cells were counted and the density of Western blot bands was determined (b and d). Both methods showed that ZBP-89 overexpression significantly reduced HDAC3 expression, compared with saline controls (*p < 0.05, b and d). The ectopic expression of ZBP-89 also significantly increased Bak expression (*p < 0.05, d). **p < 0.01, compared with saline controls.