Figure 3
From: ZBP-89 reduces histone deacetylase 3 by degrading IkappaB in the presence of Pin1
ZBP-89 stimulated IκB phosphorylation to decrease HDAC3. Cells were infected with Ad-ZBP-89. Total protein was isolated and subjected to Western blot analysis of IκB and HDAC3 (a). ZBP-89 reduced the level of IκB in a dose-dependent manner in HCC cells. The densities of IκB protein band were determined and the ratio of test (IκB) to the control (actin) was calculated (b). This ratio was significantly lower in Pin1+/+ cells than in Pin1−/− cells (*p < 0.05, **p < 0.01, paired t test, compared with respective controls.