Figure 2
From: ZBP-89 reduces histone deacetylase 3 by degrading IkappaB in the presence of Pin1
Knockdown of Pin1 expression inhibited ZBP-89-mediated reduction of HDAC3. Pin1 allele-knockout Pin1−/− and Pin1+/+ cells were infected with Ad-ZBP89 and the levels of HDAC and pHDAC were examined by Western blot (a), The densities of protein bands were determined and the ratio of test (HDAC) to the control (actin) was calculated (b). This ratio was significantly lower in Pin1+/+ cells than in Pin1−/− cells. Pin1+/+ represents Pin1+/+ MEFs, and Pin1−/− represents Pin1−/− MEFs. Cells were treated with Pin1 siRNA to inhibit Pin1 expression. The inhibition of Pin1 expression increased the levels of HDAC3 and pHDAC3 in PLC/PRF/5 cells (c). The densities of protein bands were determined to quantitate the protein levels (d). MIHA and PLC/PRF/5 cells were pretreated with Pin1 siRNA before Ad-ZBP-89 infection. Pin1 inhibition significantly inhibited ZBP-89-mediated reduction of HDAC3 (e). The up-regulated expression of Bak by ZBP-89 was also markedly suppressed by Pin1 siRNA (e). Co-IP experiments showed that Pin1 bound to HDAC3 in PLC/PRF/5 cells (f). All experiments replicated at least three times. *p < 0.05, **p < 0.01 paired t test, compared with respective controls.