Figure 1
From: ZBP-89 reduces histone deacetylase 3 by degrading IkappaB in the presence of Pin1
Reduction of HDAC3 by ZBP-89. The ectopic expression of ZBP-89 attenuated HDAC3 protein expression but not HDAC4 in MIHA, PLC/PRF/5 and HepG2 cells. The level of HDAC3 was decreased in a dose-dependent manner by ZBP-89 (a). Cell lysate was obtained to separate the cytoplasmic and nuclear fractions. The reduction of HDAC3 and pHDAC3 proteins mainly occurred in the nucleus (b). The cytoplasmic and nuclear fractions were verified using anti-actin and anti-lamin B antibodies respectively (c). RT-PCR analysis showed that the level of HDAC3 mRNA was not changed by ZBP-89 ectopic expression (d). All experiments replicated at least three times.