HUVEC proliferation and protein expressions of pro-
angiogenesis factors after lung-
A & B) Immunohistochemical stain of BrdU uptake by HUVECs (gray color). C) The statistically analytic results of BrdU+cells, p < 0.0001, * vs. control group. D, E & F) As compared with control group, the protein expression of basic-fibroblast growth factor (b-FGF) was not enhanced by Lc-MP treatment at the three time intervals (6, 12 and 24 h) of HUVEC culture. However, as compared with that of control group, the protein expression of b-FGF were significantly increased in HUVECs at time intervals of 12 and 24 h after vascular endothelial cell growth factor (VEGF) (50 ng/mL) treatment. * vs. other groups with different symbols, p < 0.001. G,
H & I) As compared with control group, the protein expression of VEGFR2 in HUVECs were significantly augmented at 6 h and 12 h intervals and maintained at 24 h following Lc-MP treatment. * vs. other groups with different symbols, p < 0.001. Additionally, this protein expression in HUVECs showed an identical pattern of b-FGF at time intervals of 12 and 24 h after VEGF (50 ng/mL) treatment. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level).