Figure 1
Upper Panel) Matrigel Assay for angiogenesis with and without Patient’s Lung cancer- derived microparticles (Lc- MPs) treatment (n =  6). A to C) After 5-hour cell culture [1.0 × 104 human umbilical vein endothelial cells (HUVECs)], Matrigel-assay angiogenesis was observed by microscopic findings (100×) in without (A) and with Lc-MPs treatment [3.0 × 105 (B) and 6.0 × 105 (C) MPs, respectively]. D) Analytical results of number of tubules (white arrows), * vs. other groups with different symbols (*, †, ‡), p < 0.001. E) Analytical results of total tubular length, * vs. other groups with different symbols (*, †, ‡), p < 0.001. F) Analytical results of cluster formation (black arrows), * vs. other groups with different symbols (*, †, ‡), p < 0.001. G) Analytical results of network formation (blue dot line), * vs. other groups with different symbols (*, †, ‡), p < 0.001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test. Lower Panel) Aortic-ring (AR) angiogenesis and nitric oxide (NO) production after lung-cancer microparticles (Lc-MPs) treatment (n = 6). H & I) By day-5 cell culturing, the angiogenesis did not differ between with and without Lc-MPs treatment. J & K) By day-12 cell culturing, the AR angiogenesis was significantly enhanced in with (K) than in without (J) Lc-MP treatment. N) Mean sprouting length, p < 0.0001, * vs. control group; O) Number of sprouts around aortic ring, p < 0.0001, * vs. control group. L & M) Immunofluorescent microscopic finding of NO production by 6-hour after cell culture; P) The statistically analytic results of fluorescent intensity of NO expression, p < 0.0001, * vs. control group.