Employment of digital gene expression profiling to identify potential pathogenic and therapeutic targets of fulminant hepatic failure

Table 1 Primer sequences used in the quantitative RT-PCR analysis

Gene	Length	Forward primers	Reverse primers
TNFα	183 bp	CCCTCCAGAAAAGACACCATG	CACCCCGAAGTTCAGTAGACAG
TNFr	172 bp	GCAAAATCCCCCATACTCAAG	AGACCTATTTGGCACTCGCA
IκBα	180 bp	CCCTTACTGGAGAGACCCGA	GCAACAGAATAGCACCGACG
iNos	215 bp	GGGAATCTTGGAGCGAGTTG	GCCTATCCGTCTCGTCCGT
TLR7	141 bp	GTTCTATGGAGAGCCGGTGATA	ATTCTTTAGATTTGGCGGCATA
Ccl3	152 bp	CACTGCCCTTGCTGTTCTTC	GGCTGCTGGTTTCAAAATAGTC
Ccl4	162 bp	TCTCTCTCCTCTTGCTCGTGG	CTGGCTTGGAGCAAAGACTG
Bid	189 bp	CAGACCTGCTGGTGTTCGG	CCTGACTTTCAGAATCTGGCTC
Bcl2	154 bp	CTGAGAGAGGCAGGCGATG	CGATGCGACCCCAGTTTAC
C5a	158 bp	CATTGCTCCTCACCATTCCA	CAGAGGCAACACAAAACCCA
Egr2	154 bp	GCCCCTTTGACCAGATGAAC	GAGAATTTGCCCATGTAAGTGAA
Fos	157 bp	TGACAGATACACTCCAAGCGG	GGGAAGCCAAGGTCATCG
Junb	182 bp	AGTTACTCCCCAGCCTCTGC	GGTACGGTCTGCGGTTCCT
Map3k5	192 bp	GCCTAAACTAAAGTGGGAACACAT	CGAAGAACTTTATTGACCGCA
β-actin	263 bp	GAGACCTTCAACACCCCAGC	ATGTCACGCACGATTTCCC

ISSN: 1479-5876