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Figure 1 | Journal of Translational Medicine

Figure 1

From: Inhibition of dipeptidyl peptidase-IV enzyme activity protects against myocardial ischemia-reperfusion injury in rats

Figure 1

Reactive oxygen species (ROS) generation in heart of live rats and pathological findings at 72 h after ischemia-reperfusion (IR) procedure. A to E) Immunofluorescent microscopic findings (400×) of 2′,7′-dichlorodihydrofluorescin diacetate (H2DCFDA) stain for identifying the ROS generation in left ventricular (LV) myocardium. F) Analytic results of fluorescent intensity, p < 0.0001, * vs. other groups with different symbols (*, †, ‡). Scale bars in right lower corner represent 20 μm. G to K) Microscopic findings (100×) of H&E stain for identifying the infarct area in LV myocardium. L) Analytic results of infarct area, p < 0.0001, * vs. other groups with different symbols (*, †, ‡). Scale bars in right lower corner represent 100 μm. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 8). Symbols (*, †, ‡) indicate significance (at 0.05 level). WT-SC = wide type sham control; WT-IR = wide type + ischemia reperfusion (IR); DDP4D-SC = dipeptidyl peptidase-IV (DPP4) deficiency sham control; DDP4D-IR = DDP4D + IR; WT-IR-Sita = wide type + IR + sitagliptin. HPF = high-power field. M and N) The Western blot results showed that the protein expression of circulating CD26, an indicator of DDP-4 activity, was remarkably suppressed in Fischer 344 rat with (N) than in without (M) sitagliptin treatment. O and P) The Western blot results showed that the protein expression of circulating CD26 in DDP-4 deficient rat (P) was lost as compared with that of circulating CD26 in Fischer 344 rat (O).

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