Table 1 Clone, dilution, and source of antibodies used in this study
From: Site-specific metabolic phenotypes in metastatic breast cancer
Antibody | Clone | Dilution | Source |
|---|---|---|---|
Molecular subtype related | Â | Â | Â |
  ER | SP1 | 1:100 | Thermo Scientific, CA, USA |
  PR | PgR | 1:50 | DAKO, Denmark |
  HER-2 | Polyclonal | 1:1500 | DAKO, Denmark |
  Ki-67 | MIB-1 | 1:150 | DAKO, Denmark |
Glycolysis related | |||
  Glut-1 | SPM498 | 1:200 | Abcam, Cambridge, UK |
  Hexokinase II | 3D3 | 1:200 | Abcam, Cambridge, UK |
  CAIX | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  MCT4 | Polyclonal | 1:100 | Santa Cruz, CA, USA |
Glutaminolysis related | |||
  GLS1 | Polyclonal | 1:50 | Abcam, Cambridge, UK |
  GDH | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  ASCT2 | Polyclonal | 1:100 | Abcam, Cambridge, UK |
Mitochondrial related | |||
  ATP synthase | 15H4C4 | 1:100 | Abcam, Cambridge, UK |
  SDHA | 2E3GC12FB2AE2 | 1:100 | Abcam, Cambridge, UK |
  SDHB | 21A11AE7 | 1:100 | Abcam, Cambridge, UK |
Serine/glycine metabolism related | Â | Â | Â |
  PHGDH | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  PSPH | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  PSAT1 | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  SHMT | Polyclonal | 1:100 | Abcam, Cambridge, UK |
  GLDC | Polyclonal | 1:100 | Abcam, Cambridge, UK |