Figure 5From: Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1 Detection of H5N1 virus by directional sandwich ELISA and comparison with the conventionally undirectional ELISA. (A) Serial concentrations (0, 10, 50, 100, 500, 1000, 5000 ng/mL) of H5N1 antigen were used for detection. The linear relationship in the range from 50 to 1000 ng/mL. The linear equation was calculated as Y = 0.0006 X + 0.1640 with an acceptable correlation coefficient of 0.9947 (R2). The data represent mean ± standard deviation from triplicate measurements. (B) The concentration of H5N1 from 0 to 1000 ng/mL to test directional and undirectional sandwich ELISA system. Error bars are standard deviations of three measurements.Back to article page