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Figure 4 | Journal of Translational Medicine

Figure 4

From: Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1

Figure 4

Specificity detection of three Nanobodies against H5N1 by ELISA. Different subtypes of influenza viruses were coated onto microtiter plates and the Nanobodies were added to incubate with them. After the reaction with the mouse anti-HA tag antibody and then with the rabbit anti-mouse IgG-alkaline phosphatase, the chromogenic solution containing bis phosphate were added and the absorbance was measured by an ELISA reader at 405 nm. The values were the means of three replicates. Nb7 and Nb93 only recognized H5N1 virus but not other subtypes.

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