Figure 3From: Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1 Purification of H5N1-specific Nanobodies. (A) Three Nanobodies encoded by different sequence were purified by immobilized metal affinity chromatography (IMAC) using a His-Select matrix. These Nanobodies were detected by coomassie brilliant blue stained SDS-PAGE. (B) Isoelectric point, molecular weight and output of three Nanobodies.Back to article page