Figure 1From: Streptavidin-biotin-based directional double Nanobody sandwich ELISA for clinical rapid and sensitive detection of influenza H5N1 Library construction. (A) The size of the library was determined by counting the number of clones after gradient dilution and the library size was 5 x 108 cfu/mL. (B) 24 colonies were randomly selected to estimate the correct insertion rate by performing PCR. The size of products was approximately 400 bp.Back to article page