Figure 6From: Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications Detection of PA by a sandwich ELISA based on BiNb48 and the HRP-coupled S-anti-PA Nb. (A) BiNb48 was eluted into 6Â mM biotin solution and analyzed by SDS-PAGE. (B) Calibration curve for PA detection. The linear relationship was in the range from 50 to 1000Â ng/mL.Back to article page