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Figure 5 | Journal of Translational Medicine

Figure 5

From: Construction of a synthetic phage-displayed Nanobody library with CDR3 regions randomized by trinucleotide cassettes for diagnostic applications

Figure 5

Comparison of the reactive sensitivity to PA and thermostability analysis of two libraries-derived anti-PA Nbs. (A) Indirect ELISA was used to compare the reactive sensitivity to PA with different gradient concentrations of anti-PA Nbs ranged from 0.001 to 10 μg/mL. The error bars represent the standard deviation derived from three independent experiments. (B) Thermostability analysis of two libraries-derived anti-PA Nbs. Three Nbs stayed at 37°C for 0, 2, 12, 24, 48, 60 and 72 h were used to perform ELISA. The activity of Nbs never stayed under 37°C was regarded as 100%. Three independent experiments were performed.

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