Figure 4
Nbs purification and ELISA mapping of the purified Nbs. (A) The recombinant Nbs were orderly eluted with 100, 250 and 500 mM imidazole solution, and the purity was verified by SDS-PAGE. (B) ELISA mapping of PA-specific Nb and NGAL-specific Nb using different PA and NGAL concentrations ranging from 0.1 to 20 μg/mL. Three independent experiments were performed.