Figure 5
Proliferation and cytokine production in DC vaccine prepared by adherence method is antigen specific. (A) Adherence DCs were co-cultured with apoptotic 3T3 (DC/ctrl) or with 3T3 infected with influenza (DC/flu) and assessed for proliferation by 3H thymidine incorporation at 24, 48 and 96 hours beyond day 8 (the day DCs would have been administered as a vaccine). Results are of triplicate wells. * indicates p < 0.05. (B) Adherence DC/ctrl and DC/flu were stained with CD8 antibody, influenza-M1 specific dextramer and IFNγ antibody. The original PBMC population was used as the comparison group. The upper panels shows CD8+ cells that are gated on M1+ staining. The lower panels show M1+ cells that are gated on IFNγ + cells.