Figure 3
Lymphocytes in Adherence DC vaccine preparations are activated and proliferating. (A) Representative flow cytometry profile of forward scatter versus side scatter of Adherence and Selected DC vaccine preparations. CD4, CD8, and CD19 antibody staining of cells in the lymphocytes gate in the Adherence DC plot is shown by histogram. (B) DCs made with the Adherence method were stained with antibodies to lymphocyte markers, CD4, CD8 or CD19 and activation markers CD40, CD83, CD69 and HLA-DR and analyzed by flow cytometry. The results shown are representative of 3 different donors.