Figure 5

Impact of PAI-1 to the decline of the angiogenic activity of ADSC obtained from the patients with CAD and CAD + DM2. (A-B) PAI-1 production by ADSC from patients with CAD and DM2 increased compared to the control group. PAI-1 gene expression in ADSC (A) and protein concentration in ADSC conditioned medium (B) are presented. Data are median and percentiles (25-75%), p-values are presented on the graphs. (C-D) Representative microphotographs of capillary-like tubes formed by EA.hy 926 endothelial cell line on Matrigel in the presence of ADSC conditioned media (patient with CAD) with (C) or without (D) PAI-1 neutralizing antibodies (5 mkg/ml). (E) Total capillary-like tubes length measurement in the presence of PAI-1 neutralizing antibodies (5 ug/ml) in ADSC conditioned medium to the standard condition (without antibodies). Data are median and percentiles (25-75%), p-value is presented on the graph.