ABE inhibits RANKL-induced osteoclastdifferentiation inbone marrow derived macrophages (BMMs). (A) BMMs were cultured in the presence of M-CSF and RANKL withor without different concentrations of ABE (0.16, 0.8, 4 μg/ml, respectively). Six days post-culture, cells were fixed with 4% paraformadehyde followed by TRAP staining. Representative images of TRAP staining of osteoclast-like cells from one of the three experiments are shown. B-C: Quantitative analysis show the mean number of TRAP-positive osteoclast-like cells (B), mean osteoclast-like cells nuclei numbers (C). Data are represented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 significantly different from Model group.