Figure 4

Proliferation of CRC cells after transfection with RFC4-siRNA assessed by the MTT and EdU assays. (A) RFC4 expression in SW480 cells following transfection with scrambled siRNA (Sc), RFC4-siRNA#1, -siRNA#2 or -siRNA#3. (B) RFC4 expression in DLD1 cells following transfection with scrambled siRNA (Sc), RFC4-siRNA#1, -siRNA#2 or -siRNA#3. (C) and (D) Western blots paralleling the treatments in panels A and B, respectively. (E) Cell growth in control and SW480 cells transfected with scrambled or RFC4-siRNA#2 as assessed by the MTT assay. (F) Cell growth in control and DLD1 cells transfected with scrambled or RFC4-siRNA#1 as assessed by the MTT assay. Transfection with RFC4 siRNA decreases cell proliferation in SW480 and DLD1 cells. (G) EdU incorporation assays for DNA synthesis in DLD1 cells transfected with scrambled siRNA or RFC4-siRNA. There is a significant reduction in the proliferation of DLD1 cells transfected with RFC-siRNA. *P <0.05.