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Table 1 Phenotypic characterization and CMV-specific CTL isolation of unmanipulated PBMCs and the non-adherent fraction

From: CMV-specific T cell isolation from G-CSF mobilized peripheral blood: depletion of myeloid progenitors eliminates non-specific binding of MHC-multimers

  Unmanipulated PBMCs Non-adherent fraction p
  Leukocyte subpopulations  
CD14+ cells 47.8% (36.0 - 55.0) 2.1% (1.3 - 6.1) 0.005
T lymphocytes (CD3+) 24.0% (18.0 - 37.1) 69.9% (31.9 - 79.2) 0.007
CTLs (CD3 + CD8+) 17.7% (6.8 - 25.1) 35.2% (23.8 - 45.2) 0.009
Helper T cells (CD3 + CD4+) 12.5% (11.4 - 19.4) 19.3% (16.6 - 29.6) 0.208
Viability (7-AAD-) 98.0% (95.6 - 98.5) 96.0% (93.4 - 97.8) 0.066
  PM staining: specific and non-specific binding  
CMV-specific CTLs (CD3 + CD8 + PM+) 0.14% (0.06 - 0.62) 0.65% (0.24 - 1.51) 0.003
Non-specific PM binding (CD8-PM+) 0.56% (0.41 - 0.86) 0.16% (0.12 - 0.37) 0.003
  CMV-specific CTL isolation  
Purity 20.8% (6.9 - 61.7) 76.0% (32.7 - 83.7) 0.043
Yield 38.6% (32.9 - 44.8) 42.1% (23.4 - 84.1) 0.893
  1. Cell subsets, CMV-specific CTLs and non-specific PM staining were analyzed in the unmanipulated PBMC sample and in the non-adherent cells (n = 11). CMV-specific CTLs were isolated from unmanipulated PBMCs and non-adherent fraction, and purity and yield of the obtained cellular product were determined (n = 5). Comparison was done with the Wilcoxon signed-rank test and significance level was fixed to p < 0.05.
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