Figure 4

miR-200c regulated the growth and metastasis of bladder cancer by directly targeting to BMI-1 and E2F3. A: miR-200c directly targeted BMI-1. miR-200c directly targeted E2F3. B: Relationship between BMI-1 and miR-200c in the mRNA level. 16 tissue specimens were tested to measure the relationship between BMI-1 mRNA and miR-200c level, and found inverse correlation between BMI-1 and miR-200c. C and D: luciferase activity of pGL3-BMI-1-3’-UTR, pGL3-E2F3-3’-UTR reporter in indicated cells cotransfected with indicated oligonucleotides. E: Western blotting of BMI-1, E-cadherin, N-cadherin, Vimentin, P14, P16, and E2F3 expression in indicated cells. β-tubulin was used as a loading control.