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Figure 2 | Journal of Translational Medicine

Figure 2

From: miR-200c Inhibits invasion, migration and proliferation of bladder cancer cells through down-regulation of BMI-1 and E2F3

Figure 2

Up-regulated miR-200c inhibited proliferation, migration and invasion in bladder cancer cells. A: Entivirus carrying miR-200c plasmid and the control plasmid were persistently co-transfected into UMUC-3 and T24 cells. Measurements by real-time RT-PCR of miR-200c confirmed our success of transduction and were obviously higher than the control group in both cell lines. B: CCK-8 assays revealed cell growth differences of indicated cell lines. C: Colony formation assay in T24 and UMUC-3 cells. D: Measurement of in vitro cell migration by “wound-healing” assay. Representative pictures (left) and quantification (right) for same single spot in indicated cell lines. E: Transwell migration assays in indicated engineered cell lines. F: Transwell invasion assays in indicated engineered cell lines. Data are presented as mean ± SD from 3 independent experiments. *P < 0.05; **P < 0.01. ***P < 0.001; DAPI, 4’, 6-diamidino-2-phenylindole.

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