Figure 4

Indirect effects of lung cancer cells on peripheral blood mononuclear cells (PBMCs) measured during the co-culture of lung cancer cells (A549) with PBMCs from healthy donors in a transwell model. A549 cells were planted in the lower chamber of the transwell and stimulated with LPS at 100 and 500 ng/ml or vehicle for 24 hrs. PBMCs from healthy donors were then added to the upper chamber of the transwell for co-culture for 48 hrs. A: frequency of CD4⁺ T cells in total PBMCs, B: frequency of CD19⁺ B cells in total PBMCs, C: frequency of Tregs in CD4⁺ T cells, D: frequency of CD45RA⁺Tregs in CD4⁺ T cells, E: frequency of CD19⁺CD24hiCD27⁺ B cells in CD19⁺ B cells; (F) Frequency of CD19⁺IL-10⁺ B cells in CD19⁺ B cells. * stand for p values less than 0.05, as compared with the control group with PBMC from healthy donors without co-culture.