miR-146a is up-regulated by IL-2 exposure alone without an activating TCR stimulation. Naïve cells were not stimulated, but following purification were cultured in the presence of 10 ng/ml IL-2. At various time points cells were harvested and miR-146a expression levels were assessed by RT-PCR. Each data point represents the average of triplicates from an individual donor. The data represents collated data from 4 donors each tested at between 1 – 4 time points.