Figure 5From: Therapeutic effect of daphnetin on the autoimmune arthritis through demethylation of proapoptotic genes in synovial cells Cell apoptosis/necrosis as measured by Annexin V/PI staining. Four experimental groups consisting of untreated cell control, 5-aza-dc at 20 μM, daphnetin at 40 μg/mL, or combination of 5-aza-dc (20 μM) and daphnetin (40 μg/mL) for 72 h. Cells were then processed for Annexin V/PI staining and analyzed by flow cytometry. The results show the mean ± S.D. .of six independent experiments. ▲P < 0.05 compared with control, ●P < 0.05 compared with 5-aza-dc, ☆P > 0.05 compared with 5-aza-dc, *P < 0.05 compared with daphnetin.Back to article page