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Figure 3 | Journal of Translational Medicine

Figure 3

From: let-7b/g silencing activates AKT signaling to promote gastric carcinogenesis

Figure 3

let-7b/g targets AKT2 in gastric cancer. (A) The binding sites of let-7b/g on the AKT2 3'UTR as predicted by TargetScan (www.targetscan.org). (B) AKT2 mRNA expression was down-regulated upon ectopic let-7b/g expression (*, P < 0.05; **, P < 0.001). (C) Western blot analysis of AKT2, p-AKT (S473) and pS6 upon let-7b/g ectopic expression in AGS, NCI-N87 and MKN45 cells. (D) Relative luciferase activity in the constructs containing wild-type binding site or mutated binding site of AKT2 3'UTR after let-7b/g transfection (Wild type, the construct containing the complementary sequence of seed region; Mutation, the binding site was mutated; *, P < 0.05).

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