Figure 3
Cell viability measured by bioluminescence versus the MTS assay after treatment with TMZ. A and B: LN18 eGFP positive cells on mono-culture spheroids treated with TMZ at doses as indicated were counted by flow cytometry, and compared to measurements by MTS assay and bioluminescence assay, respectively. C and D: LN18 eGFP positive cells on co-culture spheroids with TNC-1 cells treated with TMZ at doses as indicated were counted by flow cytometry, and compared to measurements by MTS assay and bioluminescence assay, respectively. E, F, G and H: Comparison of MTS with bioluminescence intensity measurements in the LN18, A172, U251 and C6 glioma cell lines co-cultured with TNC-1 cells after treatment with TMZ at doses as indicated.