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Table 7 Product characterization after optimization of cell processing

From: Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Parameter Spec. Mean SD n Min Max
Cell concentration (WBC/ml) FIO 1.60 + 107 1.04 + 107 20 4.40 + 106 4.39 + 107
Lymphocytes (%) ≤ 30 62 12 20 41 83
Monocytes (%) ≤ 8 14 4 20 8 23
Granulocytes (%) ≤ 55 24 10 20 6 49
Platelet concentration (PLT/ml) FIO 2.66 + 107 2.42 + 107 20 2.50 + 106 9.60 + 107
PLT/WBC ≤ 10 1.75 0.90 20 0.39 3.84
RBC concentration (RBC/ml) FIO 2.23 + 107 1.32 + 107 16 1.00 + 107 6.30 + 107
RBC/WBC FIO 1.67 0.41 16 0.77 2.27
Hematocrit (%) ≤ 3 0.29 0.18 15 0.10 0.85
CD45+/CD34+ (%) FIO 2.98 1.90 18 0.80 7.74
CD45+/CD133+ (%) FIO 0.83 1.32 18 0.02 4.26
CD133+ among CD34+ cells (%) FIO 53 10 6 40 68
CD45+/CD184+ (%) FIO 34 15 9 10 57
EPC/106 WBC (CD34+/CD133+/CD309+) FIO 44 21 5 12 60
Cell viability (%) ≥ 70 90 5 18 79 98
CFC (colonies/106 cells) FIO 6 998 4 363 7 1 417 14 400
CFU-F (colonies/106 cells) FIO 56 40 7 11 131
CFU-EC (colonies/106 cells) FIO 31 19 12 6 61
Invasion (invasion index) FIO 47 18 12 25 85
  1. WBC: White Blood Cells; PLT: Platelets; RBC: Red Blood Cells; EPC: Endothelial Progenitor Cells; CFC: Colony Forming Cells; CFU-F: Colony Forming Units-Fibroblasts; CFU-EC: Colony Forming Units-Endothelial Cells; FIO: For information only.
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