Table 7 Product characterization after optimization of cell processing

Cell concentration (WBC/ml)

FIO

1.60 + 10⁷

1.04 + 10⁷

20

4.40 + 10⁶

4.39 + 10⁷

Lymphocytes (%)

≤ 30

62

12

20

41

83

Monocytes (%)

≤ 8

14

4

20

8

23

Granulocytes (%)

≤ 55

24

10

20

6

49

Platelet concentration (PLT/ml)

FIO

2.66 + 10⁷

2.42 + 10⁷

20

2.50 + 10⁶

9.60 + 10⁷

PLT/WBC

≤ 10

1.75

0.90

20

0.39

3.84

RBC concentration (RBC/ml)

FIO

2.23 + 10⁷

1.32 + 10⁷

16

1.00 + 10⁷

6.30 + 10⁷

RBC/WBC

FIO

1.67

0.41

16

0.77

2.27

Hematocrit (%)

≤ 3

0.29

0.18

15

0.10

0.85

CD45⁺/CD34⁺ (%)

FIO

2.98

1.90

18

0.80

7.74

CD45⁺/CD133⁺ (%)

FIO

0.83

1.32

18

0.02

4.26

CD133⁺ among CD34⁺ cells (%)

FIO

53

10

6

40

68

CD45⁺/CD184⁺ (%)

FIO

34

15

9

10

57

EPC/10⁶ WBC (CD34⁺/CD133⁺/CD309⁺)

FIO

44

21

5

12

60

Cell viability (%)

≥ 70

90

5

18

79

98

CFC (colonies/10⁶ cells)

FIO

6 998

4 363

7

1 417

14 400

CFU-F (colonies/10⁶ cells)

FIO

56

40

7

11

131

CFU-EC (colonies/10⁶ cells)

FIO

31

19

12

6

61

Invasion (invasion index)

FIO

47

18

12

25

85