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Table 3 Preliminary experiments: low speed washing improves platelets' removal (n = 14)

From: Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Washing speed % PLT removal % MNC yield CFC/106cells CFU-F/106cells Invasion index
400 ± g 91.59 ± 8.74 13.43 ± 9.26 11 310 ± 2 847 18 ± 10 37 ± 14
100 ± g 95.11 ± 4.12** 10.69 ± 8.29** 12 620 ± 2 581* 18 ± 10 47 ± 7
  1. Mononuclear cells were isolated from 14 bone marrow samples through density gradient on standard or low density Ficoll-Paque, then washed in Dulbecco's phosphate buffered saline without Ca2+ and Mg2+; for the last washing step, each sample was divided in 2 aliquots that were centrifuged in parallel at 400 ± g and 100 ± g, as indicated.
  2. PLT: Platelets; MNC: Mononuclear cells (lymphocytes +μmonocytes); CFC: Colony Forming Cells; CFU-F: Colony Forming Units-Fibroblast.
  3. Data are reported as mean ± standard deviation. *p < 0.05; **p < 0.01, paired T-test.
  4. CFC assay was performed on 8 product batches only, CFU-F and Invasion assays on 4 product batches only.
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