Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Table 3 Preliminary experiments: low speed washing improves platelets' removal (n = 14)

Washing speed	% PLT removal	% MNC yield	CFC/10⁶cells	CFU-F/10⁶cells	Invasion index
400 ± g	91.59 ± 8.74	13.43 ± 9.26	11 310 ± 2 847	18 ± 10	37 ± 14
100 ± g	95.11 ± 4.12**	10.69 ± 8.29**	12 620 ± 2 581*	18 ± 10	47 ± 7

Mononuclear cells were isolated from 14 bone marrow samples through density gradient on standard or low density Ficoll-Paque, then washed in Dulbecco's phosphate buffered saline without Ca²⁺ and Mg²⁺; for the last washing step, each sample was divided in 2 aliquots that were centrifuged in parallel at 400 ± g and 100 ± g, as indicated.
PLT: Platelets; MNC: Mononuclear cells (lymphocytes +μmonocytes); CFC: Colony Forming Cells; CFU-F: Colony Forming Units-Fibroblast.
Data are reported as mean ± standard deviation. *p < 0.05; **p < 0.01, paired T-test.
CFC assay was performed on 8 product batches only, CFU-F and Invasion assays on 4 product batches only.

ISSN: 1479-5876