|
Centrifugation parameters
|
Gradient medium
|
% PLT removal
|
% GRA removal
|
% MNC yield
|
|---|
|
400 × g, 20°C, 30'
|
Standard Ficoll-Paque (1.077 g/L)
|
91.49 ± 7.14
|
94.55 ± 3.75
|
21.03 ± 16.14
|
|
Low Density Ficoll-Paque (1.073 g/L)
|
91.84 ± 7.82
|
97.28 ± 2.12**
|
12.12 ± 9.45*
|
- Five sternal bone marrow samples were processed in parallel using the same centrifugation parameters but different gradient media, as indicated. Data are reported as mean ± standard deviation. *p < 0.05; **p < 0.01, paired T-test.
-
PLT: Platelets; GRA: Granulocytes; MNC: Mononuclear cells (lymphocytes +μmonocytes).
