Skip to main content

Table 2 Preliminary experiments: using a low density gradient medium ameliorates granulocytes' removal (n = 5)

From: Bone marrow-derived cells for cardiovascular cell therapy: an optimized GMP method based on low-density gradient improves cell purity and function

Centrifugation parameters Gradient medium % PLT removal % GRA removal % MNC yield
400 × g, 20°C, 30' Standard Ficoll-Paque (1.077 g/L) 91.49 ± 7.14 94.55 ± 3.75 21.03 ± 16.14
Low Density Ficoll-Paque (1.073 g/L) 91.84 ± 7.82 97.28 ± 2.12** 12.12 ± 9.45*
  1. Five sternal bone marrow samples were processed in parallel using the same centrifugation parameters but different gradient media, as indicated. Data are reported as mean ± standard deviation. *p < 0.05; **p < 0.01, paired T-test.
  2. PLT: Platelets; GRA: Granulocytes; MNC: Mononuclear cells (lymphocytes +μmonocytes).
\