Figure 2

Product stability. Nine sternal bone marrow samples were processed according to the new manufacturing method. The resulting product batches entered a stability program: they were stored at 10°C, and tested at 0-6-20-24 hours, for the following parameters: (A) Cell concentration. (B) Cell viability. (C) Percentage of mononuclear cells (MNC) (lymphocytes +μmonocytes). (D) Percentage of CD34⁺ cells. (E) Colony Forming Cell assay (CFC), to detect hematopoietic precursor cells. (F) Colony Forming Unit-Fibroblast assay (CFU-F), to detect mesenchymal precursor cells. (G) Invasion assay, to detect cells with invasion capacity. (H) Colony Forming Unit-Endothelial Cell assay (CFU-EC), to detect cells with angiogenic potential Data are expressed as relative values in respect to time 0. Each dot represents a single batch (identified by a distinct color), while black dashes indicate mean values. $\mathrm{Relative}\mathrm{value}\left(\%\right)=\frac{\mathrm{value}\mathrm{at}\mathrm{the}\mathrm{indicated}\mathrm{time}\mathrm{point}-\mathrm{value}\mathrm{at}\mathrm{time}0}{\mathrm{value}\mathrm{at}\mathrm{time}0}\mathrm{x}100$.