New manufacturing method: improved product immunophenotype and potency. Sternal bone marrow samples were processed according to the current or the new manufacturing method, as indicated, then tested as follows: (A) Immunophenotype, to detect CD34+ and CD133+ stem cells. (B) Colony Forming Cell assay (CFC), to detect hematopoietic precursor cells. (C) Colony Forming Unit-Fibroblast assay (CFU-F), to detect mesenchymal precursor cells. (D) Invasion assay, to detect cells with invasion capacity. Data are expressed as mean values; error bars represent standard deviations; *p < 0.05; **p < 0.01, unpaired T-test.