Figure 5

Bicalutamide-treatment reduced PSA protein expression in intra-femoral PCSD1 tumors in immunohistochemical analysis. Immunohistochemical analysis of PSA was performed on sections from intra-femoral and sub-cutaneous PCSD1 tumors and counterstained with hematoxylin. Hematoxylin and eosin (H&E) and anti-PSA stains counterstained with hematoxylin were performed on fixed cryosections for intra-femoral tumors and FFPE sections of sub-cutaneous tumors. A.) PSA immunostaining intensity was greater in vehicle than in bicalutamide treated intra-femoral (IF) PCSD1 tumors. Magnification was 200×. B.) PSA staining was greater in vehicle than bicalutamide- treated PCSD1 intra-femoral tumors. Quantitative digital immunohistochemical analysis of PSA immunostaining intensity was performed and plotted as Total PSA stained area/Total area of analysis. Error bars denote standard deviation.